The GUIDANCE server

guide-tree based alignment confidence

Select Algorithm

Warning: GUIDANCE is not suitable for alignments of very few sequences.
As a rule of thumb, use HoT for less than 8 sequences.

Type your sequences (FASTA format only)

Upload your sequences file (FASTA format only)    

Sequences Type: Amino Acids    Nucleotides    Codons

Select the MSA algorithm    
Warning: PRANK is significantly more time consuming. MAFFT is the fastest.

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Your email address will be used to update you the moment the results are ready.

Job title (Optional)

Enter a descriptive job title for your GUIDANCE query

                                      Show Example Results

Advanced Options

Number of bootstrap repeats    

Output order:
  Same as input

Genetic Code:   

Advanced MAFFT Options:
Refinement Strategy

Advanced PRANK Options:
Trust insertions (+F)

                                      Show Example Results