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Running Parameters:

Sequences = vpu.fas
MSA Algorithm: MAFFT
Advanced Alignment Parameters: --reorder

Running Messages:

GUIDANCE calculation is finished:


Output Files:

NOTE: The initial result for this example is an overall unreliable alignment. So almost all of the alignment is removed when removing columns using the default cutoff of 0.93. It is recommended to use the "Remove unreliable sequences" option first (below), which re-aligns the sequences after removing some, and then use "Remove unreliable columns" option for the second alignment.