GUIDANCE Server - a web server for assessing alignment confidence score

Compute multiple sequence alignment confidence score

Select algorithm

More details about each algorithm in the overview section.

GUIDANCE is not suitable for alignments of very few sequences.
As a rule of thumb, use GUIDANCE2 or HoT for less than 8 sequences.

Type your sequences (FASTA format only)

Large strings should be uploaded as files.

Or, upload your sequences file (FASTA format only)

Select sequences type

Amino Acids

Nucleotides

Codons

Select input type

Select the MSA algorithm

Warning: PRANK is significantly more time-consuming. MAFFT is the fastest.

Please enter your email address (optional)

Your email address will be used to update you the moment the results are ready.

Job title (Optional)

Enter a descriptive job title for your GUIDANCE query.

Advanced Options

Number of bootstrap repeats

Output order

Same as input

Aligned

Genetic Code:

Advanced MAFFT Options:

Max-Iterate

Pairwise alignment method

Advanced PRANK Options:
Trust insertions (+F)