The GUIDANCE server

guide-tree based alignment confidence

Select Algorithm

Warning: GUIDANCE is not suitable for alignments of very few sequences.
As a rule of thumb, use HoT for less than 8 sequences.

Type your sequences (FASTA format only)

OR
Upload your sequences file (FASTA format only)    

Sequences Type: Amino Acids    Nucleotides    Codons

Select the MSA algorithm    
Warning: PRANK is significantly more time consuming. MAFFT is the fastest.


Please enter your email address (Optional)     
Your email address will be used to update you the moment the results are ready.


Job title (Optional)

Enter a descriptive job title for your GUIDANCE query



             
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Advanced Options

Number of bootstrap repeats    


Output order:
  Same as input
  Aligned

Genetic Code:   

Upload MSA file for evaluation (FASTA format only)    
Use caution with this feature. Please see the Overview section

Advanced MAFFT Options:
Max-Iterate
Refinement Strategy

Advanced PRANK Options:
Trust insertions (+F)




             
                                      Show Example Results